Application of chemometric analysis based on physicochemical and chromatographic data for the differentiation origin of plant protection products containing chlorpyrifos

The aim of this study was to investigate the similarities and dissimilarities between the pesticide samples in form of emulsifiable concentrates (EC) formulation containing chlorpyrifos as active ingredient coming from different sources (i.e., shops and wholesales) and also belonging to various series. The results obtained by the Headspace Gas Chromatography–Mass Spectrometry method and also some selected physicochemical properties of examined pesticides including pH, density, stability, active ingredient and water content in pesticides tested were compared using two chemometric methods. Applicability of simple cluster analysis and also principal component analysis of obtained data in differentiation of examined plant protection products coming from different sources was confirmed. It would be advantageous in the routine control of originality and also in the detection of counterfeit pesticides, respectively, among commercially available pesticides containing chlorpyrifos as an active ingredient.     

Carbon exchange between ecosystems and atmosphere in the Czech Republic is affected by climate factors

By comparing five ecosystem types in the Czech Republic over several years, we recorded the highest carbon sequestration potential in an evergreen Norway spruce forest (100%) and an agroecosystem (65%), followed by European beech forest (25%) and a wetland ecosystem (20%). Because of a massive ecosystem respiration, the final carbon gain of the grassland was negative. Climate was shown to be an important factor of carbon uptake by ecosystems: by varying the growing season length (a 22-d longer season in 2005 than in 2007 increased carbon sink by 13%) or by the effect of short- term synoptic situations (e.g. summer hot and dry days reduced net carbon storage by 58% relative to hot and wet days). Carbon uptake is strongly affected by the ontogeny and a production strategy which is demonstrated by the comparison of seasonal course of carbon uptake between coniferous (Norway spruce) and deciduous (European beech) stands.     

Concentration,sources and risk assessment of PAHs in bottom sediments

Environmental Science and Pollution Research - The aims of the study were to investigate the concentration, sources and ecological risk of PAHs (polycyclic aromatic hydrocarbons) in bottom...     

Optimized yeast-based in vitro bioassay for determination of estrogenic and androgenic activity of hydroxylated / methoxylated metabolites of BDEs / CBs and related lipophilic organic pollutants

ABSTRACT

Persistent organic pollutants (POPs) are known to show endocrine disrupting (ED) activity, including interactions with hormone receptors. The aim of this work was to develop a bioassay applicable for evaluation of ED potency of highly lipophilic metabolites of POPs. To that end, a yeast-based bio-assay protocol was used. Estrogenic / androgenic activity of some native brominated biphenyl ethers (BDEs) / chlorinated biphenyls (CBs), and their hydroxylated / methoxylated metabolites was assessed. Since data (including potency compared to reference native hormones) obtained using different protocols vary, the possibility that yeast transforms POPs into some more potent compounds was first checked; it seems that no such transformation is important from the test applicability standpoint. The developed method was sensitive with EC50 values 6.5*10^?11 M and 4.5*10^?9 M calculated for E2 and DHT, respectively. Both CBs and BDEs show weak estrogenic activity negatively correlated with the degree of their halogenation, but their metabolites are significantly more potent xenohormones. 4-OH-2,2′,4′,6′-TeCB was the most potent estrogen receptor (ER) agonist among all tested compounds; its activity was only 1,000 times lower than that of native E2.     

The relationship between metal composition,phenolic acid and flavonoid content in Imleria badia from non-polluted and polluted areas

ABSTRACT

The aim of this study was to determine the elemental composition, phenolic content and composition and antioxidant properties of Imleria badia (Fr.) Vizzini (former names Boletus badius (Fr.) Fr., and Xerocomus badius (Fr.) E.-J. Gilbert) fruiting bodies collected from sites with different levels of pollution. Imleria badia was relatively tolerant to soil contamination with toxic elements and was able to grow in As, Cd, Hg and Pb concentrations exceeding 15, 2.9, 0.4 and 77 mg kg^?1, respectively. The concentration of elements in soil was reflected in the element content in I. badia. The fruiting bodies from polluted sites exhibited significantly higher content of all the analyzed elements. Among 21 individual phenolic compounds only protocatechiuc and caffeic acids, and quercetin were determined in fruiting bodies of I. badia. The differences between the concentration of the quantified phenolic compounds and the total flavonoid content in fruiting bodies of I. badia from unpolluted and polluted sites were not significant. However, the greatest total phenolic content was found in fruiting bodies from the polluted areas. The antioxidative capacity of mushrooms collected from heavily polluted sites was lower than those growing in unpolluted areas. The concentrations of some metals in soil and fruiting soil were positively correlated with phenolic content and IC₅₀.     

Seasonal variability of polychlorinated biphenyls (PCBs) and polychlorinated diphenyl ethers (PBDEs) congener profiles in butter in Poland: Dietary risk evaluation

Various statistical methods have been employed to analyse in details seasonal diversification of polychlorinated biphenyl (PCB)/polybrominated diphenyl ether (PBDE) congener profiles found in butter fat. The variability of the PCB/PBDE congener profiles indicates the presence of various sources of the milk fat contamination. The obtained results suggest that the environmental chemical background has the highest share in the contamination sources pattern. Ion trap mass spectrometry coupled to high-resolution gas chromatography with semi-permeable membrane dialysis sample cleanup was used for determination of PCBs and PBDEs in milk fat. Determined butter fat PCB profiles were similar to the profiles characteristic for Aroclor 1254 technical mixture. Our data indicate that dietary intake of PCB/PBDE with milk and milk products may be estimated to be about 717.5 pg kg b.w.^?1 day^?1 for six-indicator PCBs, 0.329 (equivalent toxicity, TEQ) pg kg b.w.^?1 day^?1 for 12 DL PCBs and 50 pg kg b.w.^?1 day^?1 for PBDEs (sum of 14 congeners).     

Identification of volatiles from Pinus silvestris attractive for Monochamus galloprovincialis using a SPME-GC/MS platform

Introduction

A myriad of volatile organic compounds (VOCs) released by terrestrial vegetation plays an important role in environmental sciences. A thorough chemical identification of these species at the molecular level is essential in various fields, ranging from atmospheric chemistry to ecology of forest ecosystems. In particular, the recognition of VOCs profiles in a context of plant?Cinsect communication is a key issue for the development of forest protection tools.

Purpose

This work was aimed at the development of a simple, robust and reliable method for the identification of volatiles emitted from plant materials, which can attract or deter pest insects. Specifically, volatiles emitted from the bark of Pinus sylvestris were studied, which might attract the black pine sawyer beetle Monochamus galloprovincialis??a serious pest of the tree and a vector of a parasitic nematode Bursaphelenchus xylophius.

Method

The volatiles from bark samples were collected using a solid-phase micro-extraction technique, and subsequently analysed by gas-chromatography/mass-spectrometry (GC/MS). The characterisation of the volatile fraction was based on the comparison with data in mass spectral libraries, and in most cases, with the available authentic standards. The identified compounds were screened against the available entomological data to select insect attractors.

Results

The identified components included terpenes (??-pinene, ?-3-carene, and para-cymenene), oxygenated terpenes (??-terpineol and verbenone), sesquiterpenes (??-longipinene, longifolene, E-??-farnesene, ??-cadinene and pentadecane), and diterpenes (manoyl oxide and (+)-pimaral). Of these, longifolene and (+)-pimaral are of particular interest as plausible attractors for the M. galloprovincialis beetle that might find application in the construction of insect bait traps.     

Accuracy Assessment of NOAA Gridded Daily Reference Evapotranspiration for the Texas High Plains

The National Oceanic and Atmospheric Administration (NOAA) provides daily reference evapotranspiration (ET_ref) maps for the contiguous United States using climatic data from North American Land Data Assimilation System (NLDAS). This data provides large‐scale spatial representation of ET_ref, which is essential for regional scale water resources management. Data used in the development of NOAA daily ET_ref maps are derived from observations over surfaces that are different from short (grass — ET_os) or tall (alfalfa — ET_rs) reference crops, often in nonagricultural settings, which carries an unknown discrepancy between assumed and actual conditions. In this study, NOAA daily ET_os and ET_rs maps were evaluated for accuracy, using observed data from the Texas High Plains Evapotranspiration (TXHPET) network. Daily ET_os, ET_rs and the climatic data (air temperature, wind speed, and solar radiation) used for calculating ET_ref were extracted from the NOAA maps for TXHPET locations and compared against ground measurements on reference grass surfaces. NOAA ET_ref maps generally overestimated the TXHPET observations (1.4 and 2.2 mm/day ET_os and ET_rs, respectively), which may be attributed to errors in the NLDAS modeled air temperature and wind speed, to which reference ET_ref is most sensitive. Therefore, a bias correction to NLDAS modeled air temperature and wind speed data, or adjustment to the resulting NOAA ET_ref, may be needed to improve the accuracy of NOAA ET_ref maps.     

Identifying and Evaluating a Suitable Index for Agricultural Drought Monitoring in the Texas High Plains

Drought is a complex and highly destructive natural phenomenon that affects portions of the United States almost every year, and severe water deficiencies can often become catastrophic for agricultural production. Evapotranspiration (ET) by crops is an important component in the agricultural water budget; thus, it is advantageous to include ET in agricultural drought monitoring. The main objectives of this study were to (1) conduct a literature review of drought indices with a focus to identify a simple but simultaneously adequate drought index for monitoring agricultural drought in a semiarid region and (2) using the identified drought index method, develop and evaluate time series of that drought index for the Texas High Plains. Based on the literature review, the Standardized Precipitation‐Evapotranspiration Index (SPEI) was found to satisfy identified constraints for assessing agricultural drought. However, the SPEI was revised by replacing reference ET with potential crop ET to better represent actual water demand. Data from the Texas High Plains Evapotranspiration network was used to calculate SPEIs for the major irrigated crops. Trends and magnitudes of crop‐specific, time‐series SPEIs followed crop water demand patterns for summer crops. Such an observation suggests that a modified SPEI is an appropriate index to monitor agricultural drought for summer crops, but it was found to not account for soil water stored during the summer fallow period for winter wheat.     

Biodegradation of polyethylene films with prooxidant additives

Prooxidant additives represent a promising solution to the problem of the environment contamination with polyethylene film litter. Prooxidants accelerate photo- and thermo-oxidation and consequent polymer chain cleavage rendering the product apparently more susceptible to biodegradation. The question not fully resolved remains the biodegradation itself, its mechanism and especially the factors influencing the time-frame in which it can occur. The presented review is aimed to provide comprehensible information for both microbiologists and polymer scientists, who need participate in the research leading to an understanding of the microorganism action on the oxidized polyethylene and to design of new materials.